Someone assembled this video of Famous Comedians Dealing With Hecklers. I have seen many comics do this and the all-time world class champ was Sam Kinison one night in the big room at the Comedy Store.
A guy in the audience was there with a group of friends, all somewhat tipsy. The guy briefly declared himself a participant in Sam's act and for a few minutes, Sam bantered back and forth with him. Then Sam started a long set piece — the kind which one should not interrupt — and when the guy kept yelling things out, Mr. Kinison decided it was time to shut him up.
In fact, I think a little light went off in Sam's brain and he decided the time had come to not only shut the guy up but to drive him from the room in tears. Sam started with the exact same line you'll hear him use in this not-safe-for-workplace video but he did not stop where the clip stops. He continued on the same topic for some time, discussing various sex acts — some I'd never heard of — performed on or by the heckler's mother.
The guy in the audience realized he was outgunned and shut up. Sam kept at him. The guy in the audience apologized. Sam yelled, "You're sorry? Like your mother was sorry when…" and he listed various anatomically-impossible feats the mother had supposedly attempted.
The heckler finally had had enough of it and he got up and started to walk out. Sam jumped down from the stage and started to follow him out, yelling more and more about what an inept whore the guy's mother was. When Sam reached the limit of his microphone cord, he threw down the mike and continued outside. His voice, as you might remember, carried. We were actually sitting there in the Comedy Store main room listening to the headliner standing outside on Sunset Boulevard screaming at a departing audience member that his mother was wasn't worth the fifty cents she charged for sex. In the meantime, the hecker's friends awkwardly gathered up their things and fled from the room, exiting via a route which they hoped would allow them to avoid Sam.
About a minute later, Sam returned from outside, pausing to pick up the microphone as he returned to the stage. He waited out a huge ovation from the audience, then he grinned at us and said, very politely, "Okay…so anyone else want to fuck with me?" Amazingly, no one did.