From around '64, this is a commercial for four talking toys from Mattel…and I might as well identify the actors who did the voices for the puppets. Mister Ed was voiced, there and on the show by former cowboy star Allan "Rocky" Lane. That's a photo of him above from his cowboy star days. Mel Blanc, of course, did the voice of Bugs Bunny. Cecil and Dishonest John were voiced by Irv Shoemaker, who did the honors on the then-current Beany & Cecil cartoon show.
More interesting is the lady you hear and see in the commercial. That's Janet Waldo, best known as the voice of Judy Jetson and Penelope Pitstop, among other characters. We've mentioned Janet often here — a lovely lady who's had an amazing career in radio and live-action TV as well as cartoons. She did this commercial around a half-century ago and via some secret power that I wish I had, she still doesn't look or sound all that much different.